PURIFICATION AND PROPERTIES OF MANNANASE FROM BACILLUS-SUBTILIS

被引：31
作者：
MENDOZA, NS
ARAI, M
KAWAGUCHI, T
YOSHIDA, T
JOSON, LM
机构：
[1] UNIV OSAKA PREFECTURE, COLL AGR, DEPT AGR CHEM, SAKAI, OSAKA 591, JAPAN
[2] DEPT SCI & TECHNOL, INST IND TECHNOL DEV, MANILA, PHILIPPINES
[3] OSAKA UNIV, FAC ENGN, SUITA, OSAKA 565, JAPAN
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1994年 / 10卷 / 05期
关键词：
BACILLUS-SUBTILIS; MANNAN; BETA-MANNANASE;
D O I：
10.1007/BF00367665
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Extracellular mannanase from Bacillus subtilis NM-39, an isolate from Philippine soil, was purified about 240-fold with a yield of 7.3% by ammonium sulphate fractionation, DEAE-Toyopearl chromatography and Sephacryl S-200 gel filtration. Its M(r) was 38 kDa and it had a pI of 4.8 and optimum activity at pH 5.0 and 55-degrees-C. It was stable at pH 4 to 9 and below 55-degrees-C. The amino acid composition of the enzyme was in the order Gly > Glx > Ser and Asx > Ala.
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页码：551 / 555
页数：5
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