KINETIC-ANALYSIS OF PLASMINOGEN ACTIVATION BY STAPHYLOKINASE PLASMINOGEN COMPLEX IN THE PRESENCE OF FIBRIN

被引：14
|
作者：
OKADA, K [1 ]
YUASA, H [1 ]
HAGIYA, Y [1 ]
FUKAO, H [1 ]
UESHIMA, S [1 ]
MATSUO, O [1 ]
机构：
[1] KINKI UNIV,SCH MED,DEPT PHYSIOL,OSAKA 589,JAPAN
来源：
THROMBOSIS RESEARCH | 1994年 / 76卷 / 02期
关键词：
SAK PLG COMPLEX; SK PLG COMPLEX; PLASMINOGEN ACTIVATION; FIBRIN;
D O I：
10.1016/0049-3848(94)90188-0
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Staphylokinase (SAK) expresses plasminogen activator activity by forming a complex with plasminogen. In order to elucidate the mechanism for the expression of enzymatic activity of the complex, a cross-linked staphylokinase/plasminogen (SAK/plg) complex was produced with disuccinimidyl suberate, and its enzymatic characteristics were compared with those of a streptokinase/plasminogen (SK/plg) complex. SAK/plg complex and SK/plg complex showed a band with a molecular weight of 110 kDa and 140 kDa by SDS-PAGE under non-reduced condition, respectively. Both complexes exhibited plasminogen activator activity in a concentration-dependent manner on fibrin film and synthetic chromogenic substrate assay. The kinetic analysis of enzymatic activity of both complexes was performed. The plasminogen activator activity of SAK/plg complex was enhanced about 5-fold in the presence of FCB-2. However, SK/plg complex showed only 1.7-fold increase in the presence of FCB-2. These findings indicate that the SAK/plg complex reacts with fibrin, and efficient plasminogen activation is induced on fibrin surface.
引用
收藏
页码：181 / 191
页数：11
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