SUBUNIT DYNAMICS IN ESCHERICHIA-COLI PREPROTEIN TRANSLOCASE

被引：61
作者：
JOLY, JC
LEONARD, MR
WICKNER, WT
机构：
[1] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,LOS ANGELES,CA 90024
[2] UNIV CALIF LOS ANGELES,DEPT BIOL CHEM,LOS ANGELES,CA 90024
[3] DARTMOUTH COLL,SCH MED,DEPT BIOCHEM,HANOVER,NH 03755
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 11期
关键词：
SECY; SECE; BAND; 1; SUBUNIT EXCHANGE; EPITOPE TAG;
D O I：
10.1073/pnas.91.11.4703
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
SecY, SecE, and band 1 copurify as the SecY/E integral membrane domain of Escherichia coli preprotein translocase. To measure the in vivo association of these polypeptides and assay possible exchange, plasmid-borne secY and secE genes were placed under control of the ara regulon and fused to DNA encoding the influenza hemagglutinin epitope. Cells were incubated with [S-35]methionine, grown for a ''chase'' period, and then induced with arabinose to express epitope-tagged, nonradioactive SecY and SecE. Both the wild-type and epitope-tagged polypeptides assembled into functional, heterotrimeric SecY/E complex. However, immunoprecipitation with antibody to the epitope tag did not cross-precipitate radiolabeled SecY or SecE. Thus, these subunits normally associate stably in vivo.
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收藏
页码：4703 / 4707
页数：5
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