DETECTION OF RNA ON NORTHERN BLOTS BY NEGATIVE STAINING WITH AURINTRICARBOXYLIC ACID

被引：8
作者：
OBERBAUMER, I
SPETH, C
机构：
[1] Max-Planck-Institut für Biochemie
来源：
ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 01期
关键词：
D O I：
10.1016/0003-2697(90)90257-A
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aurintricarboxylic acid (ATA) is a well-known inhibitor of RNA and DNA modifying enzymes and was suggested as a potent RNase inhibitor for preparation of RNA (Hallick et al., 1977, Nucleic Acids Res. 4, 3055-3064). We show that ATA is a very useful stain for detecting RNA on Northern blots and slot blots although it did not fully protect purified RNA in concentrated solution against RNase A. © 1990.
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页码：77 / 79
页数：3
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