BINDING OF TRANSFER-RNA TO THE RIBOSOMAL A-SITES AND P-SITES PROTECTS 2 DISTINCT SETS OF NUCLEOTIDES IN 16 S RIBOSOMAL-RNA

被引：275
作者：
MOAZED, D
NOLLER, HF
机构：
[1] Thimann Laboratories University of California, Santa Cruz
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 211卷 / 01期
关键词：
D O I：
10.1016/0022-2836(90)90016-F
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transfer RNA protects a characteristic set of bases in 16 S rRNA from chemical probes when it binds to ribosomes. We used several criteria, based on construction of well-characterized in vitro ribosome-tRNA complexes, to assign these proteins to A or P-site binding. All of these approaches lead to similar conclusions. In the A site, tRNA caused protection of G529, G530, A1492 and A1493 (strongly), and A1408 and G1494 (weakly). In the P site, the protected bases are G693, A794, C795, G926 and G1401 (strong), and A532, G966, G1338 and G1339 (weak). In contrast to what is observed for 23 S rRNA, blocking the release of EF-Tu · GDP from the ribosome by kirromycin has no detectable effect on the protection of bases in 16 S rRNA. © 1990.
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页码：135 / 145
页数：11
相关论文
共 42 条
[1]   PROTECTION OF RIBOSOMAL-RNA FROM KETHOXAL IN POLYRIBOSOMES - IMPLICATION OF SPECIFIC SITES IN RIBOSOME FUNCTION [J].
BROW, DA ;
NOLLER, HF .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 163 (01) :27-46
[2]   IDENTIFICATION OF THE SITE OF CROSS-LINKING IN 16S RIBOSOMAL-RNA OF AN AROMATIC AZIDE PHOTOAFFINITY PROBE ATTACHED TO THE 5'-ANTICODON BASE OF A-SITE BOUND TRANSFER-RNA [J].
CIESIOLKA, J ;
GORNICKI, P ;
OFENGAND, J .
BIOCHEMISTRY, 1985, 24 (18) :4931-4938
[3]  
De Groot N, 1971, Eur J Biochem, V23, P523
[4]   CROSSLINKING OF THE ANTICODON OF P-SITE BOUND TRANSFER-RNA TO C-1400 OF ESCHERICHIA-COLI 16S RNA DOES NOT REQUIRE THE PARTICIPATION OF THE 50S SUBUNIT [J].
DENMAN, R ;
COLGAN, J ;
NURSE, K ;
OFENGAND, J .
NUCLEIC ACIDS RESEARCH, 1988, 16 (01) :165-178
[5]   MUTATIONS IN 16S RIBOSOMAL-RNA DISRUPT ANTIBIOTIC - RNA INTERACTIONS [J].
DESTASIO, EA ;
MOAZED, D ;
NOLLER, HF ;
DAHLBERG, AE .
EMBO JOURNAL, 1989, 8 (04) :1213-1216
[6]   CROSS-LINKING OF THE ANTICODON OF P-SITE AND A-SITE BOUND TRANSFER-RNAS TO THE RIBOSOME VIA AROMATIC AZIDES OF VARIABLE LENGTH - INVOLVEMENT OF 16S RIBOSOMAL-RNA AT THE A-SITE [J].
GORNICKI, P ;
CIESIOLKA, J ;
OFENGAND, J .
BIOCHEMISTRY, 1985, 24 (18) :4924-4930
[7]  
GORNICKI P, 1984, J BIOL CHEM, V259, P493
[8]  
GRAJEVSKAJA RA, 1982, EUR J BIOCHEM, V128, P47
[9]   COMPARATIVE ANATOMY OF 16-S-LIKE RIBOSOMAL-RNA [J].
GUTELL, RR ;
WEISER, B ;
WOESE, CR ;
NOLLER, HF .
PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, 1985, 32 :155-216
[10]   BEHAVIOUR OF ACETYLPHENYLALANYL SOLUBLE RIBONUCLEIC ACID IN POLYPHENYLALANINE SYNTHESIS [J].
HAENNI, AL ;
CHAPEVILLE, F .
BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 114 (01) :135-+
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