EFFECT OF SOLUBLE FACTORS FROM NERVE AND MUSCLE ON ALPHA-BUNGAROTOXIN BINDING-SITES IN ISOLATED SARCOLEMMAL MEMBRANES OF THE RAT

1 Sarcolemmal membranes were isolated from normal and from denervated muscles and the specific binding of [125I]-.alpha.-bungarotoxin to the membranes was determined. 2 Cytosol prepared from either slow (soleus) or fast (extensor digitorum longus) muscle increased the toxin binding. Similar effects were seen with cytosol from muscles which had been denervated 5 to 7 days previously and with cytosol from sciatic nerve. A purified preparation of a bacterial phospholipase C also produced this effect. 3 Bathing medium in which normal or denervated muscles had been incubated, increased the toxin binding to normal membranes, but the normal bathing medium decreased the binding to membranes prepared from denervated muscles. 4 The possibility that pre-existing latent acetylcholine receptors can be activated by intracellular factors released from muscles is discussed.