INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE FROM SULFOLOBUS-SOLFATARICUS AS A MODEL FOR STUDYING THERMOSTABLE TIM-BARREL ENZYMES

被引：14

作者：

ANDREOTTI, G ^{[1
]}

TUTINO, ML ^{[1
]}

SANNIA, G ^{[1
]}

MARINO, G ^{[1
]}

CUBELLIS, MV ^{[1
]}

机构：

[1] UNIV NAPLES FEDERICO II, DEPT ORGAN & BIOL CHEM, I-80134 NAPLES, ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1994年 / 1208卷 / 02期

关键词：

INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE; ARCHAEA; TIM-BARREL; THERMOSTABILITY; (S-SOLFATARICUS);

D O I：

10.1016/0167-4838(94)90118-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Indole-3-glycerol-phosphate synthase, a thermophilic and thermostable enzyme from the archaeon Sulfolobus solfataricus, was purified and characterized. The sequence of the thermophilic enzyme was compared to the sequence of a homologous mesophilic enzyme from Escherichia coli. The secondary structure of the thermophilic enzyme was predicted taking into account the patterns of hydropathy, chain flexibility and amphipathicity and the CD spectrum. From this analysis it turned out that indole-3-glycerol-phosphate synthase from S. solfataricus can be considered a model for studying thermostable TIM-barrel enzymes. some peculiarities of the amino-acid sequence of indole-3-glycerol-phosphate synthase from S. solfataricus are discussed in relation to the thermostability of the enzyme.

引用

页码：310 / 315

页数：6

共 7 条

[1] Extremely stable indole-3-glycerol-phosphate synthase from hyperthermophilic archaeon Pyrococcus furiosus
Muhammad Arif
Naeem Rashid
Sumera Perveen
Qamar Bashir
Muhammad Akhtar
Extremophiles, 2019, 23 : 69 - 77
[2] Extremely stable indole-3-glycerol-phosphate synthase from hyperthermophilic archaeon Pyrococcus furiosus
Arif, Muhammad
Rashid, Naeem
Perveen, Sumera
Bashir, Qamar
Akhtar, Muhammad
EXTREMOPHILES, 2019, 23 (01) : 69 - 77
[3] 2.0 angstrom structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: Possible determinants of protein stability
Hennig, M
Darimont, B
Sterner, R
Kirschner, K
Jansonius, JN
STRUCTURE, 1995, 3 (12) : 1295 - 1306
[4] Folding mechanism of indole-3-glycerol phosphate synthase from Sulfolobus solfataricus:: A test of the conservation of folding mechanisms hypothesis in (βα)8 barrels
Forsyth, WR
Matthews, CR
JOURNAL OF MOLECULAR BIOLOGY, 2002, 320 (05) : 1119 - 1133
[5] Controlling Active Site Loop Dynamics in the (β/α)8 Barrel Enzyme Indole-3-Glycerol Phosphate Synthase
O'Rourke, Kathleen F.
Jelowicki, Aneta M.
Boehr, David D.
CATALYSTS, 2016, 6 (09):
[6] Relationship of Catalysis and Active Site Loop Dynamics in the (βα)8-Barrel Enzyme Indole-3-glycerol Phosphate Synthase
Schlee, Sandra
Klein, Thomas
Schumacher, Magdalena
Nazet, Julian
Merkl, Rainer
Steinhoff, Heinz-Juergen
Sterner, Reinhard
BIOCHEMISTRY, 2018, 57 (23) : 3265 - 3277
[7] THE PHOSPHOGLYCERATE KINASE AND GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENES FROM THE THERMOPHILIC ARCHAEON SULFOLOBUS-SOLFATARICUS OVERLAP BY 8-BP - ISOLATION, SEQUENCING OF THE GENES AND EXPRESSION IN ESCHERICHIA-COLI
JONES, CE
FLEMING, TM
COWAN, DA
LITTLECHILD, JA
PIPER, PW
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 233 (03): : 800 - 808

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