HYDROPHOBIC INTERACTION CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF EQU.CL, THE HORSE MAJOR ALLERGEN

被引：35

作者：

DANDEU, JP

RABILLON, J

DIVANOVIC, A

CARMILEROY, A

DAVID, B

机构：

[1] Unité d'Immuno-Allergie, Institut Pasteur, 75724 Paris

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 621卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80072-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Equ.c1, the horse (Equus caballus) major allergen, was identified in a partially purified extract obtained from a crude aqueous horse dander extract, by acetonic precipitation and a salting-out process. It wits isolated and purified by size-exclusion chromatography followed by hydrophobic interaction chromatography. Equ.c1 appeared as an almost pure protein in a fraction eluted at 1.2 M ammonium sulphate from a phenyl Superose column. It is a single peptide with a relative molecular mass of 20 000 and a pI of ca. 3.9.

引用

页码：23 / 31

页数：9

共 21 条

[1]

Creighton T. E., 1984, PROTEINS STRUCTURES, V2nd

[2] IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY FOR THE PURIFICATION OF FEL D I, A CAT MAJOR ALLERGEN, FROM A HOUSE-DUST EXTRACT [J].

DANDEU, JP ;

RABILLON, J ;

BELTRAND, MJ ;

LUX, M ;

DUVAL, R ;

DAVID, B .

JOURNAL OF CHROMATOGRAPHY, 1990, 512 :177-188

[3]

DAVID B, 1989, 13TH P C ALL CLIN IM, P422

[4] ALLERGENS OF HORSE EPITHELIUM .1. PHYSICOCHEMICAL AND IMMUNOCHEMICAL CHARACTERIZATION OF 5 DIFFERENT HORSE EPITHELIUM RAW-MATERIALS USED FOR ALLERGEN EXTRACT PREPARATION [J].

FRANKE, D ;

MAASCH, HJ ;

WAHL, R ;

SCHULTZEWERNINGHAUS, G ;

BRETTING, H .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1990, 92 (03) :309-317

[5]

GUIBERT L, 1965, ANN I PASTEUR PARIS, V108, P579

[6] HYDROPHOBIC INTERACTION CHROMATOGRAPHY - SYNTHESIS AND USE OF SOME ALKYL AND ARYL DERIVATIVES OF AGAROSE [J].

HJERTEN, S ;

ROSENGREN, J ;

PAHLMAN, S .

JOURNAL OF CHROMATOGRAPHY, 1974, 101 (02) :281-288

[7] GRADIENT AND ISOCRATIC HIGH-PERFORMANCE HYDROPHOBIC INTERACTION CHROMATOGRAPHY OF PROTEINS ON AGAROSE COLUMNS [J].

HJERTEN, S ;

YAO, KQ ;

ERIKSSON, KO ;

JOHANSSON, B .

JOURNAL OF CHROMATOGRAPHY, 1986, 359 :99-109

[8] SINGLE-STEP PURIFICATION OF F(AB')2-MU FRAGMENTS OF MOUSE MONOCLONAL-ANTIBODIES (IMMUNOGLOBULINS M) BY HYDROPHOBIC INTERACTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING TSKGEL ETHER-5PW [J].

INOUYE, K ;

MORIMOTO, K .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1993, 26 (01) :27-39

[9]

LAU KHW, 1987, J BIOL CHEM, V262, P1389

[10] ISOLATION AND PARTIAL CHARACTERIZATION OF 3 MAJOR ALLERGENS OF HORSE HAIR AND DANDRUFF [J].

LOWENSTEIN, H ;

MARKUSSEN, B ;

WEEKE, B .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1976, 51 (01) :48-67

← 1 2 3 →