BIOCONVERSION OF D-GALACTOSE INTO D-TAGATOSE

被引:94
作者
CHEETHAM, PSJ
WOOTTON, AN
机构
[1] Unilever Research, Colworth Laboratory, Sharnbrook, Bedford
关键词
GALACTOSE; TAGATOSE; GLUCOSE ISOMERASE; L-ARABINOSE-KETOL-ISOMERASE; LACTOBACILLUS-GAYONII;
D O I
10.1016/0141-0229(93)90032-W
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
D-Tagatose has potential food uses as a humectant and/or non-calorific sweetener. A variety of different microbial strains were tested for ability to produce D-tagatose from D-galactose. As a result, we have found that D-tagatose can be produced from D-galactose by the lactic acid bacterium Lactobacillus gayonii. Surprisingly, this bioconversion of a D-hexose sugar is probably carried out by an L-pentose isomerase (L-arabinose-ketol-isomerase) enzyme activity of the cells. Thus this enzyme reacts with the D-hexose isomer of its normal L-pentose substrate. Commercially available D-glucose (xylose)-ketol-isomerases did not carry out this bioconversion. Options for the optimization of this bioconversion are proposed.
引用
收藏
页码:105 / 108
页数:4
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