TRYPANOSOMA-CRUZI - CLONING AND EXPRESSION OF AN ANTIGEN RECOGNIZED BY ACUTE AND CHRONIC HUMAN CHAGASIC SERA

被引：20

作者：

PARANHOS, GS

COTRIM, PC

MORTARA, RA

RASSI, A

CORRAL, R

FREILIJ, HL

GRINSTEIN, S

WANDERLEY, J

CAMARGO, ME

DASILVEIRA, JF

机构：

[1] HOSP NINOS DR RICARDO GUTIERREZ, BUENOS AIRES, ARGENTINA

[2] UNIV ESTADUAL CAMPINAS, HOSP CLIN, BR-13100 CAMPINAS, SP, BRAZIL

[3] UNIV SAO PAULO, INST TROP MED, SAO PAULO, BRAZIL

[4] UNIV FED GOIANIA, FAC MED, GOIANIA, BRAZIL

来源：

EXPERIMENTAL PARASITOLOGY | 1990年 / 71卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

0.15 M NaCl 0.015 M Sodium citrate pH 7.0; Acute and chronic phases; Antigen; Chagas' disease; DNA sequence; IgM and IgG detection; Molecular cloning; Trypanosoma cruzi;

D O I：

10.1016/0014-4894(90)90033-9

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Here we describe the characterization of a Trypanosoma cruzi DNA sequence (clone A13) that codes for a polypeptide recognized by IgM and IgG antibodies from sera of acute and congenital chagasic patients. Antibodies to A13 antigen are also detected in the sera of chronic patients with different clinical forms of Chagas' disease, but not in sera of patients with leishmaniasis or other parasitic diseases. The antigenic determinants encoded by clone A13 are found in amastigotes and trypomastigotes of several T. cruzi strains, but not in the noninfective epimastigotes. The DNA sequence of the recombinant clone reveals one open reading frame encoding 251 amino acids without tandemly repeated sequences. Our data suggest that the A13 antigen may be useful for the development of serodiagnostic procedures. © 1990.

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页码：284 / 293

页数：10

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