KINETICS OF THE INHIBITION OF HUMAN-LEUKOCYTE ELASTASE BY EGLIN FROM THE LEECH HIRUDO-MEDICINALIS

被引：45

作者：

BAICI, A ^{[1
]}

SEEMULLER, U ^{[1
]}

机构：

[1] UNIV MUNICH, SURG CLIN, DEPT CLIN CHEM & CLIN BIOCHEM, D-8000 MUNICH 2, GERMANY

来源：

BIOCHEMICAL JOURNAL | 1984年 / 218卷 / 03期

关键词：

D O I：

10.1042/bj2180829

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rate constants for the inhibition of human leukocyte elastase by eglin from the leech Hirudo medicinalis were determined by using a pre-steady-state kinetic approach. The kon and koff for complex-formation and dissociation were 1 .times. 106 per M per s and 8 .times. 10-4 per s, respectively. Ki was calulated as the ratio koff/kon = 8 .times. 10-10 M; the binding of eglin to elastase was reversible and the inhibition mechanism was of the fully competitive type. The mechanistic properties of the system and the biological significance of the rate constants are discussed.

引用

页码：829 / 833

页数：5

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