THE REQUIREMENT OF ARCA AND FNR FOR PEAK EXPRESSION OF THE CYD OPERON IN ESCHERICHIA-COLI UNDER MICROAEROBIC CONDITIONS

被引：104

作者：

FU, HA ^{[1
]}

IUCHI, S ^{[1
]}

LIN, ECC ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,200 LONGWOOD AVE,BOSTON,MA 02115

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 226卷 / 1-2期

关键词：

MICROAEROBIOSIS; CYD; ARCA; FNR; ESCHERICHIA-COLI;

D O I：

10.1007/BF00273605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcriptional regulation of cyd, encoding the cytochrome d complex for O2 scavenging, was studied in Escherichia coli by monitoring phi(cyd-lac) expression under atmospheres containing 0-21% O2. Peak expression of this operon occurred under microaerobic conditions. Mutations in arcA (a trans-acting regulatory gene involved in aerobic respiration) greatly lowered phi(cyd-lac) expression under all conditions. Mutations in fnr (a trans-acting regulatory gene involved in anaerobic respiration) shifted the pattern of phi(cyd-lac) expression, lowering the microaerobic and aerobic expression. In an arcA-fnr double mutant, phi(cyd-lac) expression became insignificant, irrespective of the availability of O2. It thus appears that the expression of the phi(cyd-lac) operon is under dual control by the two pleiotropic activators, ArcA and Fnr, which interact to give the peak microaerobic expression.

引用

页码：209 / 213

页数：5

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