A DISRUPTION-REPLACEMENT APPROACH FOR THE TARGETED INTEGRATION OF FOREIGN GENES IN HANSENULA-POLYMORPHA

被引：28

作者：

AGAPHONOV, MO ^{[1
]}

BEBUROV, MY ^{[1
]}

TERAVANESYAN, MD ^{[1
]}

SMIRNOV, VN ^{[1
]}

机构：

[1] CARDIOL RES CTR, INST EXPTL CARDIOL, MOSCOW 121552, RUSSIA

来源：

YEAST | 1995年 / 11卷 / 13期

关键词：

HANSENULA POLYMORPHA; GENE DISRUPTION; GENE REPLACEMENT; PROTEIN SECRETION; HUMAN URINARY PLASMINOGEN ACTIVATOR;

D O I：

10.1002/yea.320111304

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A system has been developed which allows the selection of integrative transformants with replacement of the Hansenula polymorpha methanol oxidase gene (MOX) with expression cassettes carrying heterologous gene under the control of the MOX promoter. The system is convenient for comparison of the expression levels of different constructs integrated into the same locus of the H. polymorpha genome. This system was used to compare the secretion levels of human urinary plasminogen activator, the secretion of which was directed by different signal sequences.

引用

页码：1241 / 1247

页数：7

共 26 条

[1] ISOLATION AND CHARACTERIZATION OF THE LEU2 GENE OF HANSENULA-POLYMORPHA [J].

AGAPHONOV, MO ;

POZNYAKOVSKI, AI ;

BOGDANOVA, AI ;

TERAVANESYAN, MD .

YEAST, 1994, 10 (04) :509-513

[2] THE FIBRIN PLATE METHOD FOR ESTIMATING FIBRINOLYTIC ACTIVITY [J].

ASTRUP, T ;

MULLERTZ, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1952, 40 (02) :346-351

[3] AN EFFECTIVE TRANSFORMATION METHOD FOR HANSENULA-POLYMORPHA [J].

BERARDI, E ;

THOMAS, DY .

CURRENT GENETICS, 1990, 18 (02) :169-170

[4] PLASMID REORGANIZATION DURING INTEGRATIVE TRANSFORMATION IN HANSENULA-POLYMORPHA [J].

BOGDANOVA, AI ;

AGAPHONOV, MO ;

TERAVANESYAN, MD .

YEAST, 1995, 11 (04) :343-353

[5]

BROACH JR, 1979, GENE, V8, P121, DOI 10.1016/0378-1119(79)90012-X

[6]

CESARENI G, 1987, GENETIC ENG PRINCIPL, V4, P875

[7] EXPRESSION OF THE ALPHA-GALACTOSIDASE FROM CYAMOPSIS-TETRAGONOLOBA (GUAR) BY HANSENULA-POLYMORPHA [J].

FELLINGER, AJ ;

VERBAKEL, JMA ;

VEALE, RA ;

SUDBERY, PE ;

BOM, IJ ;

OVERBEEKE, N ;

VERRIPS, CT .

YEAST, 1991, 7 (05) :463-473

[8] HETEROLOGOUS GENE-EXPRESSION IN HANSENULA-POLYMORPHA - EFFICIENT SECRETION OF GLUCOAMYLASE [J].

GELLISSEN, G ;

JANOWICZ, ZA ;

MERCKELBACH, A ;

PIONTEK, M ;

KEUP, P ;

WEYDEMANN, U ;

HOLLENBERG, CP ;

STRASSER, AWM .

BIO-TECHNOLOGY, 1991, 9 (03) :291-295

[9] HIGH-LEVEL EXPRESSION OF FOREIGN GENES IN HANSENULA-POLYMORPHA [J].

GELLISSEN, G ;

JANOWICZ, ZA ;

WEYDEMANN, U ;

MELBER, K ;

STRASSER, AWM ;

HOLLENBERG, CP .

BIOTECHNOLOGY ADVANCES, 1992, 10 (02) :179-189

[10] THE METHYLOTROPHIC YEASTS [J].

GLEESON, MA ;

SUDBERY, PE .

YEAST, 1988, 4 (01) :1-15

← 1 2 3 →