BIOGENESIS OF VACCINIA - ISOLATION AND CHARACTERIZATION OF A SURFACE COMPONENT THAT ELICITS ANTIBODY SUPPRESSING INFECTIVITY AND CELL-CELL FUSION

被引：90

作者：

STERN, W ^{[1
]}

DALES, S ^{[1
]}

机构：

[1] PUBL HLTH RES INST CITY NEW YORK INC, DEPT CYTOBIOL, NEW YORK, NY 10016 USA

来源：

VIROLOGY | 1976年 / 75卷 / 01期

关键词：

D O I：

10.1016/0042-6822(76)90022-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Tubular structures that cover the surface of vaccinia were dissociated from pure virus and isolated in a polymeric state. These surface tubules (ST) appeared on polyacrylamide gels as a single polypeptide with a MW of 58,000. Rabbit antiserum prepared agains surface tubules neutralized virus infectivity [assayed in mouse fibroblast L2 cells] and suppressed [HeLa] cell fusion. Pure ST blocked the neutralizing capacity of rabbit antiserum against whole virus and the ST. The biological functions of ST in cell-virus and cell-cell interactions are discussed.

引用

页码：232 / 241

页数：10

共 25 条

[1]

Blough HA, 1973, ADV LIPID RES, V11, P267

[2] SOLUBLE ANTIGENS OF VACCINIA-INFECTED MAMMALIAN CELLS .3. RELATION OF EARLY AND LATE PROTEINS TO VIRUS STRUCTURE [J].

COHEN, GH ;

WILCOX, WC .

JOURNAL OF VIROLOGY, 1968, 2 (05) :449-&

[3] UPTAKE AND DEVELOPMENT OF REOVIRUS IN STRAIN L CELLS FOLLOWED WITH LABELED VIRAL RIBONUCLEIC ACID AND FERRITIN-ANTIBODY CONJUGATES [J].

DALES, S ;

GOMATOS, PJ ;

HSU, KC .

VIROLOGY, 1965, 25 (02) :193-&

[4] UPTAKE AND DEVELOPMENT OF VACCINIA VIRUS IN STRAIN L CELLS FOLLOWED WITH LABELED VIRAL DEOXYRIBONUCLEIC ACID [J].

DALES, S .

JOURNAL OF CELL BIOLOGY, 1963, 18 (01) :51-+

[5] AN ELECTRON MICROSCOPE STUDY OF EARLY ASSOCIATION BETWEEN 2 MAMMALIAN VIRUSES AND THEIR HOSTS [J].

DALES, S .

JOURNAL OF CELL BIOLOGY, 1962, 13 (02) :303-+

[6] CONTROLLED DEGRADATION OF VACCINIA VIRIONS IN VITRO - AN ELECTRON MICROSCOPIC STUDY [J].

EASTERBROOK, KB .

JOURNAL OF ULTRASTRUCTURE RESEARCH, 1966, 14 (5-6) :484-+

[7] EARLY VIRUS PROTEIN-SYNTHESIS IN VACCINIA VIRUS-INFECTED CELLS [J].

ESTEBAN, M .

JOURNAL OF GENERAL VIROLOGY, 1973, 19 (MAY) :201-216

[8] LOCALIZATION OF NUCLEOTIDE PHOSPHOHYDROLASE ACTIVITY WITHIN VACCINIA [J].

GOLD, P ;

DALES, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 60 (03) :845-&

[9] PHOSPHOTUNGSTATE STAINING OF VACCINIA VIRUS [J].

HARRIS, WJ ;

WESTWOOD, JC .

JOURNAL OF GENERAL MICROBIOLOGY, 1964, 34 (03) :491-&

[10] STUDIES ON STRUCTURAL PROTEINS OF VACCINIA VIRUS .2. KINETICS OF SYNTHESIS OF INDIVIDUAL GROUPS OF STUCTURAL PROTEINS [J].

HOLOWCZAK, JA ;

JOKLIK, WK .

VIROLOGY, 1967, 33 (04) :726-+

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