PURIFICATION OF DOMOIC ACID FROM TOXIC BLUE MUSSELS (MYTILUS-EDULIS) AND PHYTOPLANKTON

被引：9
作者：
NIJJAR, MS
GRIMMELT, B
BROWN, J
机构：
[1] Department of Anatomy and Physiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 02期
基金：
加拿大自然科学与工程研究理事会;
关键词：
D O I：
10.1016/0378-4347(91)80177-E
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Domoic acid was the primary neurotoxin in blue mussel (Mytilus edulis) that caused poisoning in humans. Further research showed that the algae, Nitzschia pungens, was the source of this toxin. In this study, a method for the extraction and purification of domoic acid from contaminated mussels and phytoplankton was developed. Domoic acid was extracted from these sources by treatment with a mixture of chloroform and methanol (1:2, v/v). The resulting extract was subjected to ultrafiltration through a PM1 Millipore filter, followed by repeated high-performance liquid chromatography on a reversed-phase column. The purity and yield of domoic acid prepared by this method are compared with two previously described methods of extraction. The current method is relatively simple, rapid, and results in improved recovery with comparable purity of domoic acid.
引用
收藏
页码：393 / 406
页数：14
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