RAPID SEQUENCING OF OLIGONUCLEOTIDES BY HIGH-RESOLUTION MASS-SPECTROMETRY

被引：171

作者：

LITTLE, DP ^{[1
]}

CHORUSH, RA ^{[1
]}

SPEIR, JP ^{[1
]}

SENKO, MW ^{[1
]}

KELLEHER, NL ^{[1
]}

MCLAFFERTY, FW ^{[1
]}

机构：

[1] CORNELL UNIV,BAKER LAB,DEPT CHEM,ITHACA,NY 14853

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1994年 / 116卷 / 11期

关键词：

D O I：

10.1021/ja00090a039

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Electrospray ionization coupled with Fourier-transform mass spectrometry routinely achieves sufficiently high mass accuracy (less than or equal to 20 ppm) and resolving power (10(5)) to provide base composition and sequence information from oligonucleotides as large as 25 bases. Nozzle skimmer (NS) dissociation yields fragment anions that describe, consistent with the McLuckey mechanism, the complete sequence of 8-14-base oligomers and extensive sequence information for a 21-mer and a 25-mer. The spectrum from collisionally activated dissociation of an 8-mer is closely similar to its NS spectrum. Several spectra per minute can be measured, predicting a key role for ESI/MS in nucleotide sequence verification.

引用

页码：4893 / 4897

页数：5

共 48 条

[1] OPEN TRAPPED ION CELL GEOMETRIES FOR FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY [J].