RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS OF 16S RIBOSOMAL-RNA GENES IN THE DIFFERENTIATION OF FAST-GROWING MYCOBACTERIAL SPECIES

被引：22

作者：

DOMENECH, P ^{[1
]}

MENENDEZ, MC ^{[1
]}

GARCIA, MJ ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,FAC MED,DEPT MED PREVENT,E-28029 MADRID,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 116卷 / 01期

关键词：

MYCOBACTERIUM; 16S RIBOSOMAL-RNA GENE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

D O I：

10.1016/0378-1097(94)90101-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA from several species of fast growing mycobacteria displayed a characteristic restriction fragment length polymorphism (RFLP) pattern when hybridizated to a Mycobacterium fortuitum 16S rRNA gene fragment. The resulting patterns were identical when comparing different strains belonging to the same species. The RFLP results were consistent with those obtained by DNA-DNA hybridization studies. Using this approach, we have been able to identify the number of copies for 16S rRNA genes in several fast-growing mycobacteria.

引用

页码：19 / 24

页数：6

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