A 2.5 KB NCOI FRAGMENT OF OGURA RADISH MITOCHONDRIAL-DNA IS CORRELATED WITH CYTOPLASMIC MALE-STERILITY IN BRASSICA CYBRIDS

被引:85
作者
BONHOMME, S
BUDAR, F
FERAULT, M
PELLETIER, G
机构
[1] Laboratoire de Biologie Cellulaire, INRA Centre de Versailles, Versailles Cedex, F-78026, Route de Saint-Cyr
关键词
BRASSICA; OGURA CYTOPLASMIC MALE-STERILITY; CYBRIDS; MITOCHONDRIAL DNA;
D O I
10.1007/BF00326293
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Spontaneous reversion to fertility was studied in the progeny of a cytoplasmic male-sterile (CMS) Brassica napus cybrid containing recombinant B. napus/Ogura radish mitochondrial genomes. This reversion is concomitant with the disappearance of a 2.5 kb NcoI fragment present in the mitochondrial DNA of Ogura radish, and of CMS cybrids derived from plants carrying Ogura cytoplasm, and absent in the mitochondrial genome of normal Brassicas and fertile cybrids. This specific fragment hybridizes to a 1.4 kb transcript found only in male-sterile plants bearing an Ogura derived cytoplasm.
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页码:121 / 127
页数:7
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