ISOLATION OF CDNA CLONES FOR HUMAN-COMPLEMENT COMPONENT-C2

被引:74
作者
BENTLEY, DR
PORTER, RR
机构
来源
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES | 1984年 / 81卷 / 04期
关键词
D O I
10.1073/pnas.81.4.1212
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Two c[complementary]DNA clones for complement component C2 were isolated from a high-complexity human liver cDNA library by using a mixture of 64 synthetic oligonucleotides as a probe. The 400-base-pair insert of pC201 codes for a region containing the active site serine residue and the secondary substrate binding pocket of the serine protease. This part of C2 is 34% homologous to the corresponding region of the related serine protease factor B and additional similarity is evident from a number of conservative amino acid replacements in this region. The insert of pC201 was used as a specific probe in RNA transfer analysis to determine the size of the C2 mRNA as .apprxeq. 2.9 kilobases. Southern blot analysis of genomic DNA of unrelated individuals identified a single C2 locus and showed no cross-hybridization with the factor B locus.
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页码:1212 / 1215
页数:4
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