THE SESQUITERPENE LACTONE DIHYDROLEUCODIN IN TISSUE-CULTURE FROM ARTEMISIA-DOUGLASIANA

被引：3
|
作者：
PESTCHANKER, LJ
GIULIETTI, AM
PESTCHANKER, MJ
GUERREIRO, E
GIORDANO, OS
机构：
[1] Departamento de Química Orgánica, Universidad Naciomal de San Luis, 5700 San Luis, Chacabuco y Pedernera
来源：
PHYTOCHEMISTRY | 1990年 / 29卷 / 06期
关键词：
Artemisia douglasiana; Compositae; dihydroleucodin; guaianolides; tissue culture;
D O I：
10.1016/0031-9422(90)85027-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Callus tissue from seedling explants (roots, stems, leaves) and adult plants (stems and leaves) of Artemisia douglasiana were cultured on a modified Murashige-Skoog medium. Friable calli were obtained only when the following growth regulator combinations were used: gibberellic acid plus 3-indoleacetic acid (IAA), gibberellic acid plus 3-indolebutyric acid (IBA), gibberellic acid plus α-naphthaleneacetic acid (NAA). These calli produced dihydroleucodin (DHL) as the main metabolise which was determined by HPLC. Not all A. douglasiana calli have the same ability to produce DHL. Calli from leaves of seedling and adult plants produced the largest amounts of DHL. © 1982.
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页码：1853 / 1854
页数：2
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