Role of 6 integrin subunit in branching morphogenesis of fetal mouse submandibular gland : investigation by mesenchyme-free epithelial culture system

被引：10

作者：

Koyama, Noriko ^{[1
]}

Hayashi, Toru ^{[1
]}

Gresik, Edward W. ^{[2
]}

Kashimata, Masanori ^{[1
]}

机构：

[1] Asahi Univ, Sch Dent, Dept Pharmacol, 1851 Hozumi, Mizuho, Gifu 5010296, Japan

[2] CUNY, Sch Med, Dept Cell Biol & Anat, New York, NY 10031 USA

来源：

JOURNAL OF MEDICAL INVESTIGATION | 2009年 / 56卷

关键词：

alpha; 6; integrin; Branching morphogenesis; Cleft formation; Stalk elongation; Submandibular gland;

D O I：

10.2152/jmi.56.247

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The submandibular gland (SMG) of the fetal mouse is a well-studied model for the epithelial-mesenchymal interactions required for branching morphogenesis (BrM), which involves cleft formation and stalk elongation. In a previous report, we showed that alpha 6 integrin subunit is involved in BrM of this gland rudiment, since the neutralizing antibody against alpha 6 integrin subunit, GoH3, strongly inhibited branching of cultured intact E13 SMG. In this study, we investigated whether GoH3 inhibits cleft formation and/or stalk elongation during BrM of cultured mesenchyme-free epithelial rudiments of fetal SMG and also analyzed by Western blotting the levels of phosphorylation of ERK1/2, which is a signaling molecule known to regulate BrM of the fetal mouse SMG.

引用

页码：247 / 249

页数：3

共 9 条

[1] Morphogenesis [J].

Hogan, BLM .

CELL, 1999, 96 (02) :225-233

[2] INTEGRINS - VERSATILITY, MODULATION, AND SIGNALING IN CELL-ADHESION [J].

HYNES, RO .

CELL, 1992, 69 (01) :11-25

[3] DISTRIBUTION OF ALPHA-6 INTEGRIN SUBUNIT IN DEVELOPING MOUSE SUBMANDIBULAR-GLAND [J].

KADOYA, Y ;

YAMASHINA, S .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1993, 41 (11) :1707-1714

[4]

Kashimata M, 1997, DEV DYNAM, V208, P149, DOI 10.1002/(SICI)1097-0177(199702)208:2<149::AID-AJA2>3.0.CO

[5]

2-I

[6] Role of the extracellular matrix in morphogenesis [J].

Kleinman, HK ;

Philp, D ;

Hoffman, MP .

CURRENT OPINION IN BIOTECHNOLOGY, 2003, 14 (05) :526-532

[7] EGF-stimulated signaling by means of PI3K, PLCγ1, and PKC isozymes regulates branching morphogenesis of the fetal mouse submandibular gland [J].

Koyama, N ;

Kashimata, M ;

Sakashita, H ;

Sakagami, H ;

Gresik, EW .

DEVELOPMENTAL DYNAMICS, 2003, 227 (02) :216-226

[8] Signaling pathways activated by epidermal growth factor receptor or fibroblast growth factor receptor differentially regulate branching morphogenesis in fetal mouse submandibular glands [J].

Koyama, Noriko ;

Hayashi, Toru ;

Ohno, Kenji ;

Siu, Larry ;

Gresik, Edward W. ;

Kashimata, Masanori .

DEVELOPMENT GROWTH & DIFFERENTIATION, 2008, 50 (07) :565-576

[9] Laminin α5 is necessary for submandibular gland epithelial morphogenesis and influences FGFR expression through β1 integrin signaling [J].

Rebustini, Ivan T. ;

Patel, Vaishali N. ;

Stewart, Julian S. ;

Layvey, Ann ;

Georges-Labouesse, Elisabeth ;

Miner, Jefftey H. ;

Hoffman, Matthew P. .

DEVELOPMENTAL BIOLOGY, 2007, 308 (01) :15-29

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