THE MICRO WATER 1,2-DICHLOROETHANE INTERFACE AS A TRANSDUCER FOR CREATININE ASSAY

被引：38
|
作者：
OSBORNE, MD
GIRAULT, HH
机构：
[1] Institut de Chimie Physique III, Ecole Polytechnique Fédérale de Lausanne, Lausanne
来源：
MIKROCHIMICA ACTA | 1995年 / 117卷 / 3-4期
关键词：
BIOSENSOR; MICRO-ITIES; CREATININE; AMPEROMETRIC-ISE;
D O I：
10.1007/BF01244888
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Facilitated ammonium ion transfer reactions, using the crown ether dibenzo-18-crown-6, at a single microhole supported water/1,2-dichloroethane interface were used as the transducer to detect the biomolecule creatinine. The ammonium ion was produced during the enzymatic action of creatinine deiminase on creatinine. A prototype sensing device incorporating a Teflon gas permeable membrane, to exclude possible interferents, yielded a linear amperometric response to added creatinine from 20 muM to 1 mM. This range is sufficient for that required for the determination of creatinine in serum.
引用
收藏
页码：175 / 185
页数：11
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