GIBBERELLIC-ACID AND ABSCISIC-ACID COORDINATELY REGULATE CYTOPLASMIC CALCIUM AND SECRETORY ACTIVITY IN BARLEY ALEURONE PROTOPLASTS

被引:173
作者
GILROY, S [1 ]
JONES, RL [1 ]
机构
[1] UNIV CALIF BERKELEY, DEPT PLANT BIOL, BERKELEY, CA 94720 USA
关键词
CONFOCAL SCANNING OPTICAL MICROSCOPY; PLANT HORMONES; RATIO IMAGING; SECRETION;
D O I
10.1073/pnas.89.8.3591
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The effects of gibberellic acid (GA3) and abscisic acid (ABA) on the temporal and spatial dynamics of cytosolic calcium concentration ([Ca2+]i) in aleurone protoplasts of barley (Hordeum vulgare L. cv. Himalaya) were measured by using fluorescence ratio analysis and confocal microscopy. After 4-6 h of treatment, GA3 induced a sustained increase in [Ca2+]i from 50 to 150 nM in aleurone protoplasts. The increase in [Ca2+]i preceded the GA3-induced increase in alpha-amylase synthesis and secretion by 4 h. The elevation of [Ca2+]i was highest in the peripheral cytoplasm and may play a role in coordinating the secretory events there. Reducing Ca2+ levels in the incubation medium to below 500-mu-M inhibited the increase in [Ca2+]i and the GA3-induced stimulation of alpha-amylase synthesis and secretion. These data suggest that GA3 may increase [Ca2+]i by increasing the influx of Ca2+ at the plasma membrane. ABA reversed the effect of GA3 on [Ca2+]i within 3 h, 2 h before its effect on alpha-amylase production could be detected. Thus changes in [Ca2+]i may play a role in mediating the effects of GA3 and ABA on the synthesis and secretion of alpha-amylase in the aleurone cell.
引用
收藏
页码:3591 / 3595
页数:5
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