WHOLE-CELL PATCH-CLAMP RECORDINGS OF AN NMDA RECEPTOR-MEDIATED SYNAPTIC CURRENT IN RAT HIPPOCAMPAL SLICES

被引：38

作者：

RANDALL, AD ^{[1
]}

SCHOFIELD, JG ^{[1
]}

COLLINGRIDGE, GL ^{[1
]}

机构：

[1] UNIV BRISTOL, SCH MED SCI, DEPT PHARMACOL, BRISTOL BS8 1TH, AVON, ENGLAND

来源：

NEUROSCIENCE LETTERS | 1990年 / 114卷 / 02期

基金：

英国惠康基金;

关键词：

6-Cyano-7-nitroquinoxaline-2,3-dione; d-2-Amino-5-phosphonovalerate; Hippocampus; N-Methyl-d-aspartate receptor; Picrotoxin; Synaptic current;

D O I：

10.1016/0304-3940(90)90070-P

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Whole-cell patch-clamp recordings and pharmacological techniques have been used to obtain low noise recordings of 2 excitatory postsynaptic synaptic currents (termed EPSAA and EPSCB) evoked by stimulation of the Schaffer collateral-commissural pathway in rat hippocampal slices. EPSCA was blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and EPSCB was blocked by d-2-amino-5-phosphonovalerate (APV), indicating their mediation by non-N-methyl-d-aspartate (non-NMDA) and NMDA receptors, respectively. EPSCB has a slower time-course than EPSCA and its current-voltage relationship was highly non-linear with a region of negative slope conductance from -35 to -100 mV. These properties of EPSCA and EPSCB can explain their differing participation in synaptic transmission in this pathway. © 1990.

引用

页码：191 / 196

页数：6

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