BIOASSAY OF SOLUBILIZED BACILLUS-THURINGIENSIS VAR ISRAELENSIS CRYSTALS BY ATTACHMENT TO LATEX BEADS

被引：51
|
作者：
SCHNELL, DJ [1 ]
PFANNENSTIEL, MA [1 ]
NICKERSON, KW [1 ]
机构：
[1] UNIV NEBRASKA, SCH BIOL SCI, LINCOLN, NE 68588 USA
来源：
SCIENCE | 1984年 / 223卷 / 4641期
关键词：
D O I：
10.1126/science.6701520
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Solubilized crystals of B. thuringiensis var. israelensis [a biological cointrol agent] were 7000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding H2O and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8 .mu.m latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.
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页码：1191 / 1193
页数：3
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