ASSAY OF CHOLINESTERASE USING A PRO-ENHANCER OF THE LUMINOL-H2O2-HORSERADISH PEROXIDASE REACTION

2-Naphthyl acetate acts as a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction. Cholinesterase hydrolyses the bound acetyl group and produces 2-naphthol, and this compound is an enhancer of the chemiluminescent reaction. We studied the kinetics of chemiluminescent emission and the influence of 2-naphthyl acetate and cholinesterase enzyme concentrations. The cholinesterase concentration versus chemiluminescence intensity maximum was linear for cholinesterase between 0 and 181 mu U/mL, with a detection limit of 8 mu U/mL and a relative standard deviation of 9.5% (n = 3). for a sample containing 90.67 mu U/mL of cholinesterase