AMINO-ACID-SEQUENCES OF 3 ACYL-BINDING LIPID-TRANSFER PROTEINS FROM RAPE SEEDLINGS

The complete amino acid sequence of three acyl-binding/lipid-transfer proteins, AB/LTP I, AB/LTP II and AB/LTP III from germinated rape seeds were determined. AB/LTP I and AB/LTP II consist of 93 residues and the M(r) was determined as 9408 by mass spectrometry and calculated as 9406.8 from the sequence. AB/LTP III consists of 92 residues and the M(r) was determined as 9424 by mass spectrometry and calculated as 9422.8 from the sequence. The primary structures were determined by automated Edman degradations of the intact proteins and peptides obtained from digestion with trypsin and endoproteinase Asp-N and cyanogen bromide Cf-252 plasma-desorption mass spectrometry facilitated the identification and verification of peptides.