QUANTITATIVE AUTORADIOGRAPHY REVEALS DIFFERENT ANGIOTENSIN-II RECEPTOR SUBTYPES IN SELECTED RAT-BRAIN NUCLEI

被引：150

作者：

TSUTSUMI, K ^{[1
]}

SAAVEDRA, JM ^{[1
]}

机构：

[1] NIMH, CLIN SCI LAB, PHARMACOL SECT, BLDG 10, ROOM 2D-45, BETHESDA, MD 20892 USA

来源：

JOURNAL OF NEUROCHEMISTRY | 1991年 / 56卷 / 01期

关键词：

ANGIOTENSIN ANTAGONIST; SUBFORNICAL ORGAN; PARAVENTRICULAR NUCLEUS; INFERIOR OLIVE; RECEPTOR CLASSIFICATION;

D O I：

10.1111/j.1471-4159.1991.tb02602.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heterogeneity of rat brain angiotensin II receptors was revealed by quantitative autoradiography after incubation with I-125-Sar1-angiotensin II and displacement with the angiotensin II antagonists CGP 42112 A and DuP-753 and by receptor sensitivity to dithiothreitol. Receptors in areas involved in cardiovascular and fluid control-the subfornical organ, nucleus of the solitary tract, paraventricular nucleus, and area postrema-are displaced by DuP-753 with an IC50 of 1 x 10(-7) M, are sensitive to 5 mM dithiothreitol, and thus are angiotensin II type-1. Receptors in the inferior olive are displaced by CGP 42112 A (IC50, 1 x 10(-9)) but not by DuP-753 in concentrations up to 10(-4) M, are insensitive to 5 mM dithiothreitol, and thus are angiotensin II type-2.

引用

页码：348 / 351

页数：4

共 16 条

[11] FUNCTIONS OF ANGIOTENSIN IN THE CENTRAL-NERVOUS-SYSTEM [J].

PHILLIPS, MI .

ANNUAL REVIEW OF PHYSIOLOGY, 1987, 49 :413-435

[12]

SAAVEDRA JM, 1989, INT REV NEUROBIOL, V31, P257

[13] QUANTITATIVE DISTRIBUTION OF ANGIOTENSIN-II BINDING-SITES IN RAT-BRAIN BY AUTORADIOGRAPHY [J].

SAAVEDRA, JM ;

ISRAEL, A ;

PLUNKETT, LM ;

KURIHARA, M ;

SHIGEMATSU, K ;

CORREA, FMA .

PEPTIDES, 1986, 7 (04) :679-687

[14] BINDING OF ANGIOTENSIN AND ATRIAL-NATRIURETIC-PEPTIDE IN BRAIN OF HYPERTENSIVE RATS [J].

SAAVEDRA, JM ;

CORREA, FMA ;

PLUNKETT, LM ;

ISRAEL, A ;

KURIHARA, M ;

SHIGEMATSU, K .

NATURE, 1986, 320 (6064) :758-760

[15] DISTRIBUTION OF ANGIOTENSIN-II RECEPTORS IN RAT-BRAIN [J].

SIRETT, NE ;

MCLEAN, AS ;

BRAY, JJ ;

HUBBARD, JI .

BRAIN RESEARCH, 1977, 122 (02) :299-312

[16] PRELIMINARY BIOCHEMICAL-CHARACTERIZATION OF 2 ANGIOTENSIN-II RECEPTOR SUBTYPES [J].

WHITEBREAD, S ;

MELE, M ;

KAMBER, B ;

DEGASPARO, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 163 (01) :284-291

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