QUANTITATIVE AUTORADIOGRAPHY REVEALS DIFFERENT ANGIOTENSIN-II RECEPTOR SUBTYPES IN SELECTED RAT-BRAIN NUCLEI

被引：150

作者：

TSUTSUMI, K ^{[1
]}

SAAVEDRA, JM ^{[1
]}

机构：

[1] NIMH, CLIN SCI LAB, PHARMACOL SECT, BLDG 10, ROOM 2D-45, BETHESDA, MD 20892 USA

来源：

JOURNAL OF NEUROCHEMISTRY | 1991年 / 56卷 / 01期

关键词：

ANGIOTENSIN ANTAGONIST; SUBFORNICAL ORGAN; PARAVENTRICULAR NUCLEUS; INFERIOR OLIVE; RECEPTOR CLASSIFICATION;

D O I：

10.1111/j.1471-4159.1991.tb02602.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heterogeneity of rat brain angiotensin II receptors was revealed by quantitative autoradiography after incubation with I-125-Sar1-angiotensin II and displacement with the angiotensin II antagonists CGP 42112 A and DuP-753 and by receptor sensitivity to dithiothreitol. Receptors in areas involved in cardiovascular and fluid control-the subfornical organ, nucleus of the solitary tract, paraventricular nucleus, and area postrema-are displaced by DuP-753 with an IC50 of 1 x 10(-7) M, are sensitive to 5 mM dithiothreitol, and thus are angiotensin II type-1. Receptors in the inferior olive are displaced by CGP 42112 A (IC50, 1 x 10(-9)) but not by DuP-753 in concentrations up to 10(-4) M, are insensitive to 5 mM dithiothreitol, and thus are angiotensin II type-2.

引用

页码：348 / 351

页数：4

共 16 条

[1] REPEATED STRESS INCREASES THE DENSITY OF ANGIOTENSIN-II BINDING-SITES IN RAT PARAVENTRICULAR NUCLEUS AND SUBFORNICAL ORGAN [J].

CASTREN, E ;

SAAVEDRA, JM .

ENDOCRINOLOGY, 1988, 122 (01) :370-372

[2] ANGIOTENSIN-II RECEPTORS IN PARAVENTRICULAR NUCLEUS, SUBFORNICAL ORGAN, AND PITUITARY-GLAND OF HYPOPHYSECTOMIZED, ADRENALECTOMIZED, AND VASOPRESSIN-DEFICIENT RATS [J].

CASTREN, E ;

SAAVEDRA, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (02) :725-729

[3]

CHIU AT, 1989, J PHARMACOL EXP THER, V250, P867

[4] IDENTIFICATION OF ANGIOTENSIN-II RECEPTOR SUBTYPES [J].

CHIU, AT ;

HERBLIN, WF ;

MCCALL, DE ;

ARDECKY, RJ ;

CARINI, DJ ;

DUNCIA, JV ;

PEASE, LJ ;

WONG, PC ;

WEXLER, RR ;

JOHNSON, AL ;

TIMMERMANS, PBMWM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (01) :196-203

[5] DISCRIMINATION OF ANGIOTENSIN-II RECEPTOR SUBTYPES BY DITHIOTHREITOL [J].

CHIU, AT ;

MCCALL, DE ;

NGUYEN, TT ;

CARINI, DJ ;

DUNCIA, JV ;

HERBLIN, WF ;

UYEDA, RT ;

WONG, PC ;

WEXLER, RR ;

JOHNSON, AL ;

TIMMERMANS, PBMWM .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1989, 170 (1-2) :117-118

[6] INCREASED ANGIOTENSIN-II RECEPTORS IN BRAIN NUCLEI OF DOCA-SALT HYPERTENSIVE RATS [J].

GUTKIND, JS ;

KURIHARA, M ;

SAAVEDRA, JM .

AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (03) :H646-H650

[7] INCREASED CONCENTRATION OF ANGIOTENSIN-II BINDING-SITES IN SELECTED BRAIN-AREAS OF SPONTANEOUSLY HYPERTENSIVE RATS [J].

GUTKIND, JS ;

KURIHARA, M ;

CASTREN, E ;

SAAVEDRA, JM .

JOURNAL OF HYPERTENSION, 1988, 6 (01) :79-84

[8] AUTORADIOGRAPHIC LOCALIZATION OF ANGIOTENSIN-II RECEPTORS IN RAT-BRAIN [J].

MENDELSOHN, FAO ;

QUIRION, R ;

SAAVEDRA, JM ;

AGUILERA, G ;

CATT, KJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (05) :1575-1579

[9]

MUNSON PJ, 1983, METHODS ENZYMOLOGY E, V92, P453

[10] CALIBRATION OF I-125 POLYMER STANDARDS WITH I-125 BRAIN PASTE STANDARDS FOR USE IN QUANTITATIVE RECEPTOR AUTORADIOGRAPHY [J].

NAZARALI, AJ ;

GUTKIND, JS ;

SAAVEDRA, JM .

JOURNAL OF NEUROSCIENCE METHODS, 1989, 30 (03) :247-253

← 1 2 →