EFFECTS OF RELA GENE ON SYNTHESIS OF AMINOACYL-TRANSFER-RNA SYNTHETASES AND OTHER TRANSCRIPTION AND TRANSLATION PROTEINS IN ESCHERICHIA-COLI-B

The effects of a partial restriction of valyl-tRNA aminoacylation on the synthesis of aminoacyl-tRNA synthetases, ribosomal proteins and other translation and transcription proteins were examined in otherwise isogenic stringent (relA+) and relaxed (relA1) derivatives of E. coli B. The synthesis of individual ribosomal proteins, elongation factor [EF] G, and to a lesser extent EF-Tu and EF-Ts, and the valyl- and arginyl-tRNA synthetases was subject to the influence of the stringent control system. The synthesis of the .alpha. and .beta. subunits of RNA polymerase and several of the aminoacyl-tRNA synthetases is either not subject to the influence of the stringent control system or is subject to additional regulatory constraints.