HUMAN PAPILLOMAVIRUS (HPV) TYPE DISTRIBUTION AND SEROLOGICAL RESPONSE TO HPV TYPE-6 VIRUS-LIKE PARTICLES IN PATIENTS WITH GENITAL WARTS

被引:188
作者
GREER, CE
WHEELER, CM
LADNER, MB
BEUTNER, K
COYNE, MY
LIANG, H
LANGENBERG, A
YEN, TSB
RALSTON, R
机构
[1] UNIV NEW MEXICO, DEPT CELL BIOL, ALBUQUERQUE, NM 87131 USA
[2] UNIV NEW MEXICO, CTR POPULAT HLTH, ALBUQUERQUE, NM 87131 USA
[3] UNIV CALIF SAN FRANCISCO, DEPT DERMATOL, SAN FRANCISCO, CA 94143 USA
[4] UNIV CALIF SAN FRANCISCO, DEPT PATHOL, SAN FRANCISCO, CA 94143 USA
关键词
D O I
10.1128/JCM.33.8.2058-2063.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Thirty-nine patients with condylomas (12 women and 27 men) attending a dermatology clinic were tested for genital human papillomavirus (HPV) DNA and for seroprevalence to HPV type 6 (HPV6) L1 virus-like particles. The L1 consensus PCR system (with primers MY09 and MY11) was used to determine the presence and types of HPV in sample specimens. All 37 (100%) patients with sufficient DNA specimens were positive for HPV DNA, and 35 (94%) had HPV6 DNA detected at the wart site. Three patients (8%) had HPV11 detected at the wart site, and one patient had both HPV6 and -11 detected at the wart site. Thirteen additional HPV types were detected among the patients; the most frequent were HPV54 (8%) and HPV58 (8%). Baculovirus-expressed HPV6 L1 virus-like particles were used in enzyme-linked immunosorbent assays to determine seroprevalence among the patients with warts. Seronegativity was defined by a control group of 21 women who were consistently PCR negative for HPV DNA. Seroprevalence was also determined for reference groups that included cytologically normal women who had detectable DNA from either HPV6 or HPV16 and women with HPV16-associated cervical intraepithelial neoplasia. Among the asymptomatic women with HPV6, only 2 of 9 (22%) were seropositive, compared with 12 of 12 (100%) female patients with warts. A similar trend in increased HPV6 seropositivity with increased grade of disease was found with the HPV16 DNA-positive women, whose seroprevalence increased from 1 in 11 (9%) in cytologically normal women to 6 in 15 (40%) among women with cervical intraepithelial neoplasia 1 or 3. However, only 4 of 25 (16%) male patients were seropositive. No factors examined, such as age, sexual behavior, or a history of warts, were found to definitively account for the gender difference in seroresponse.
引用
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页码:2058 / 2063
页数:6
相关论文
共 51 条
  • [1] AHMED SA, 1985, AM J PATHOL, V121, P531
  • [2] GENITAL HUMAN PAPILLOMAVIRUS INFECTION IN FEMALE UNIVERSITY-STUDENTS AS DETERMINED BY A PCR-BASED METHOD
    BAUER, HM
    TING, Y
    GREER, CE
    CHAMBERS, JC
    TASHIRO, CJ
    CHIMERA, J
    REINGOLD, A
    MANOS, MM
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1991, 265 (04): : 472 - 477
  • [3] Bauer HM, 1992, DIAGNOSTIC MOL PATHO, P131
  • [4] BECKER TM, 1994, JAMA-J AM MED ASSOC, V271, P1181, DOI 10.1001/jama.271.15.1181
  • [5] IDENTIFICATION AND ASSESSMENT OF KNOWN AND NOVEL HUMAN PAPILLOMAVIRUSES BY POLYMERASE CHAIN-REACTION AMPLIFICATION, RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS, NUCLEOTIDE-SEQUENCE, AND PHYLOGENETIC ALGORITHMS
    BERNARD, HU
    CHAN, SY
    MANOS, MM
    ONG, CK
    VILLA, LL
    DELIUS, H
    PEYTON, CL
    BAUER, HM
    WHEELER, CM
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1994, 170 (05) : 1077 - 1085
  • [6] EVOLUTION OF THE ANTIBODY-RESPONSE TO HUMAN PAPILLOMAVIRUS TYPE-11 (HPV-11) IN PATIENTS WITH CONDYLOMA ACUMINATUM ACCORDING TO TREATMENT RESPONSE
    BONNEZ, W
    DARIN, C
    ROSE, RC
    TYRING, SK
    REICHMAN, RC
    [J]. JOURNAL OF MEDICAL VIROLOGY, 1993, 39 (04) : 340 - 344
  • [7] USE OF HUMAN PAPILLOMAVIRUS TYPE-11 VIRIONS IN AN ELISA TO DETECT SPECIFIC ANTIBODIES IN HUMANS WITH CONDYLOMATA ACUMINATA
    BONNEZ, W
    RIN, CD
    ROSE, RC
    REICHMAN, RC
    [J]. JOURNAL OF GENERAL VIROLOGY, 1991, 72 : 1343 - 1347
  • [8] ANTIBODY-RESPONSE TO HUMAN PAPILLOMAVIRUS (HPV) TYPE-11 IN CHILDREN WITH JUVENILE-ONSET RECURRENT RESPIRATORY PAPILLOMATOSIS (RRP)
    BONNEZ, W
    KASHIMA, HK
    LEVENTHAL, B
    MOUNTS, P
    ROSE, RC
    REICHMAN, RC
    SHAH, KV
    [J]. VIROLOGY, 1992, 188 (01) : 384 - 387
  • [9] USE OF HPV-1 CAPSIDS PRODUCED BY RECOMBINANT VACCINIA VIRUSES IN AN ELISA TO DETECT SERUM ANTIBODIES IN PEOPLE WITH FOOT WARTS
    CARTER, JJ
    HAGENSEE, MB
    LEE, SK
    MCKNIGHT, B
    KOUTSKY, LA
    GALLOWAY, DA
    [J]. VIROLOGY, 1994, 199 (02) : 284 - 291
  • [10] HPV-1 CAPSIDS EXPRESSED IN-VITRO DETECT HUMAN SERUM ANTIBODIES ASSOCIATED WITH FOOT WARTS
    CARTER, JJ
    HAGENSEE, M
    TAFLIN, MC
    LEE, SK
    KOUTSKY, LA
    GALLOWAY, DA
    [J]. VIROLOGY, 1993, 195 (02) : 456 - 462