SPECIFIC PCR DETECTION AND IDENTIFICATION OF XYLELLA-FASTIDIOSA STRAINS CAUSING CITRUS VARIEGATED CHLOROSIS

被引：142

作者：

POOLER, MR ^{[1
]}

HARTUNG, JS ^{[1
]}

机构：

[1] USDA ARS,BELTSVILLE AGR RES CTR,FRUIT LAB,BELTSVILLE,MD 20705

来源：

CURRENT MICROBIOLOGY | 1995年 / 31卷 / 06期

关键词：

D O I：

10.1007/BF00294703

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically, We also identified a CVC-specific region of the X. fastidiosa genome that contains a 28-nucleotide insertion, and single base changes that distinguish CVC and grape X. fastidiosa strains. When using RAPD products to develop specific PCR primers, we found it most efficient to screen for size differences among RAPD products rather than presence/absence of a specific RAPD band.

引用

页码：377 / 381

页数：5

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