CONFORMATIONAL-CHANGES IN ADSORBED PROTEINS

被引：102

作者：

BALL, A ^{[1
]}

JONES, RAL ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,CAVENDISH LAB,CAMBRIDGE CB3 0HE,ENGLAND

来源：

LANGMUIR | 1995年 / 11卷 / 09期

关键词：

D O I：

10.1021/la00009a042

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Attenuated total reflection Fourier transform infrared (FTIR-ATR) spectroscopy was used to follow the adsorption of the globular protein lysozyme from aqueous (D2O) solution onto a silicon surface. The effect of changing temperature and/or pD on the structure of protein in the adsorbed layer is observed and compared with the effect of these changes on concentrated solutions. We show that the same spectral changes as those occurring in the heat-set gelation of concentrated solutions of globular proteins can also be observed in adsorbed proteins, but at lower temperatures. We suggest that this is due to the presence of the surface having the effect of promoting molecular structural rearrangements.

引用

页码：3542 / 3548

页数：7

共 35 条

[1]

ANDRADE J, 1985, SURFACE INTERFACIAL, V2

[2] THE BEHAVIOR OF SOME MODEL PROTEINS AT SOLID LIQUID INTERFACES .1. ADSORPTION FROM SINGLE PROTEIN SOLUTIONS [J].