HIGHLY FREQUENT APPEARANCE OF TETRAPLOIDY IN REGENERATED PLANTS, A UNIVERSAL PHENOMENON, IN TISSUE-CULTURES OF MELON (CUCUMIS-MELO L)

被引:58
作者
EZURA, H
AMAGAI, H
YOSHIOKA, K
OOSAWA, K
机构
[1] NATL INST AGROBIOL RESOURCES,TSUKUBA,IBARAKI 305,JAPAN
[2] HOKKAIDO UNIV,FAC AGR,SAPPORO,HOKKAIDO 060,JAPAN
关键词
TETRAPLOID; CUCUMIS-MELO; SOMATIC EMBRYO; ADVENTITIOUS SHOOT; SHOOT PRIMORDIA;
D O I
10.1016/0168-9452(92)90117-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The frequency of tetraploidy in melon plants (Cucumis melo L.) regenerated from somatic embryos, adventitious shoots, shoot primordia and in plants propagated from axillary buds was compared. Tetraploids were found in plants regenerated from somatic embryos, adventitious shoots and shoot primordia, and the frequency of tetraploids in the three culture methods was 31%, 30% and 4%, respectively. No tetraploidy was observed in plants propagated from axillary buds. The highly frequent appearance of tetraploidy in regenerated plants appears to be a universal phenomenon in tissue culture of melon.
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页码:209 / 213
页数:5
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