INVOLVEMENT OF OMPF DURING RECEPTION AND TRANSLOCATION STEPS OF COLICIN-N ENTRY

被引：27

作者：

BOURDINEAUD, JP ^{[1
]}

FIEROBE, HP ^{[1
]}

LAZDUNSKI, C ^{[1
]}

PAGES, JM ^{[1
]}

机构：

[1] CNRS,CTR BIOCHIM & BIOL MOLEC,31 CHEMIN JOSEPH AIGUIER,BP 71,F-13402 MARSEILLE 9,FRANCE

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 10期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb00551.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

[125I]‐colicin N binds to OmpF receptor sites (70000 per cell) with an average Kassoc of 3.2 × 106 M−1 at 23°C. Monoclonal antibody directed against a cell‐surface‐exposed epitope of OmpF is able to compete with the binding of the colicin in vitro and also to protect against colicin N in vivo. OmpF is an absolute requirement for colicin N uptake. OmpC cannot serve as a substitute for OmpF during translocation across the outer membrane under receptor bypass conditions, which is in contrast to colicin A. Colicin N does not cross‐react with various monoclonal antibodies directed against colicin A. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1737 / 1743

页数：7

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