DETERMINATION OF SELENIUM BY FLOW-INJECTION ANALYSIS BASED ON THE SELENIUM(IV)-CATALYZED REDUCTION OF 3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYL-2H TETRAZOLIUM BROMIDE

被引：11

作者：

AOYAMA, E ^{[1
]}

KOBAYASHI, N ^{[1
]}

SHIBATA, M ^{[1
]}

NAKAGAWA, T ^{[1
]}

TANAKA, H ^{[1
]}

机构：

[1] KYOTO PHARMACEUT UNIV,KYOTO 607,JAPAN

来源：

ANALYTICAL SCIENCES | 1991年 / 7卷 / 01期

关键词：

SELENIUM; FLOW INJECTION ANALYSIS; CATALYTIC REDUCTION; CATALYTIC ANALYSIS;

D O I：

10.2116/analsci.7.103

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel flow injection method has been developed for the determination of selenium in biological samples. The method is based on a selective catalytic effect of Se(IV) on the reduction of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT). The calibration line ranged over 1.3 pmol to 1.2 nmol of selenite with a detection limit of 0.63 pmol. Interference from the copper ion could be eliminated by an on-line adsorption of Cu on an ODS column as a Cu-bathocuproine disulfate chelate. The utility of the method was demonstrated by determining the selenium content in a standard sample.

引用

页码：103 / 107

页数：5

共 21 条

[1] SEMIAUTOMATED METHOD FOR DETERMINATION OF SELENIUM IN GEOLOGICAL-MATERIALS USING A FLOW-INJECTION ANALYSIS TECHNIQUE [J].