CIRCULAR-DICHROISM STUDIES OF CALICHEAMICIN-DNA INTERACTION - EVIDENCE FOR CALICHEAMICIN-INDUCED DNA CONFORMATIONAL CHANGE

被引：30
|
作者：
KRISHNAMURTHY, G [1 ]
DING, WD [1 ]
OBRIEN, L [1 ]
ELLESTAD, GA [1 ]
机构：
[1] AMER CYANAMID CO,DIV MED RES,LEDERLE LABS,PEARL RIVER,NY 10965
来源：
TETRAHEDRON | 1994年 / 50卷 / 05期
关键词：
D O I：
10.1016/S0040-4020(01)80622-0
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Evidence from circular dichroism studies suggests that the binding of calicheamicin to DNA induces an optically detectable conformational change of B-form DNA. CD titration of a dodecamer containing a TCCT binding/cleavage site with calicheamicin gamma(1)(I) the aromatized calicheamicin epsilon brings about a decrease primarily in the ellipticity of the positive 270 nm signal. Binding constants for calicheamicin gamma(1)(I) and epsilon with this dodecamer were estimated to be 9x10(5) and 5x10(4) M(-1), respectively, based on analyses of the CD changes.
引用
收藏
页码：1341 / 1349
页数：9
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