PRELIMINARY-REPORT ON A SIMPLE NEW ASSAY FOR DNA-PROTEIN CROSS-LINKS AS A BIOMARKER OF EXPOSURES EXPERIENCED BY WELDERS

被引：21

作者：

COSTA, M

ZHITKOVICH, A

TAIOLI, E

TONIOLO, P

机构：

[1] Institute of Environmental Medicine, New York University Medical Center, New York, NY

来源：

JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH | 1993年 / 40卷 / 2-3期

关键词：

D O I：

10.1080/15287399309531789

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A method originally developed to detect topoisomerase DNA complexes has been adapted to determine DNA-protein cross-links formed in cells following their exposure in vitro and in vivo to cross-linking agents. A preliminary study on welders and controls has been carried out to assess the feasibility of this assay to detect human exposure to chromium (Cr) and nickel (Ni) that are associated with increased DNA-protein cross-links. The percentage of cross-link was significantly higher among welders (1.85% +/- 1.14) than among controls (1.17% +/- 0.46; p = .01) Cross-links were not affected by age, weight, or smoking status. The assay developed has substantial advantages over previous methods, being considerably more simple, inexpensive, and sensitive.

引用

页码：217 / 222

页数：6

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