INDUCTION OF MUTATIONS IN MOUSE FM3A CELLS BY TREATMENT WITH RIBOFLAVIN PLUS VISIBLE-LIGHT AND ITS POSSIBLE RELATION WITH FORMATION OF 8-HYDROXYGUANINE (7,8-DIHYDRO-8-OXOGUANINE) IN DNA

被引：22
作者：
BESSHO, T
TANO, K
NISHIMURA, S
KASAI, H
机构：
[1] UNIV OCCUPAT & ENVIRONM HLTH,INST IND ECOL SCI,DEPT ENVIRONM ONCOL,1-1 ISEIGAOKA,YAHATANISHI KU,KITAKYUSHU,FUKUOKA 807,JAPAN
[2] NATL CANC CTR,RES INST,DIV BIOL,TOKYO 104,JAPAN
[3] MERCK RES LABS,BANYU TSUKUBA RES INST,TSUKUBA,IBARAKI 30033,JAPAN
来源：
CARCINOGENESIS | 1993年 / 14卷 / 05期
关键词：
D O I：
10.1093/carcin/14.5.1069
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Photosensitized formation of 8-hydroxyguanine (oh8Gua; 7,8-dihydro-8-oxoguanine, oxo8Gua) in cellular DNA and its repair was examined in mouse FM3A cells. oh8Gua was generated 7-fold above the background level by 10 min irradiation with visible light in the presence of riboflavin (160 muM) and repaired to .60 - 70% of initial level after 2 h post-incubation. By this treatment, mutation frequency that was measured by counting ouabain-resistant colonies, was increased 7-fold above the background frequency. The accumulation of unrepaired oh8Gua in DNA generated by treatment with riboflavin plus visible light correlates to induction of mutations. Thus, oh8Gua will contribute to the mutation induced by visible light as well as spontaneous mutations.
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页码：1069 / 1071
页数：3
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