MODIFIED SERONEUTRALIZATION ASSAY FOR MEASLES-VIRUS ANTIBODY DETECTION

被引：10
|
作者：
NATES, SV [1 ]
REY, GY [1 ]
GIORDANO, MO [1 ]
ZAPATA, MT [1 ]
DEPETRIS, A [1 ]
BOSHELL, J [1 ]
机构：
[1] MINIST SALUD,INST NACL SALUD,SANTAFE BOTOTA,COLOMBIA
来源：
RESEARCH IN VIROLOGY | 1994年 / 145卷 / 01期
关键词：
MEASLES; SERONEUTRALIZATION; DETECTION; MODIFIED ASSAY; SPECIFICITY; SENSITIVITY; PROTECTIVE ANTIBODIES;
D O I：
10.1016/S0923-2516(07)80006-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We describe a simple, inexpensive, sensitive and specific modified seroneutralization assay for use on a wide scale to screen for the presence of measles antibodies. After only one reaction (1 h) and a 3-day incubation period at 37 degrees C, the test can be easily read using a rapid, inexpensive methylene blue/phenol staining procedure. The determination of protective antibody titres by modified and standard seroneutralization methods was strongly correlated (corr. coff. = 0.98).
引用
收藏
页码：45 / 49
页数：5
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