MODIFIED SERONEUTRALIZATION ASSAY FOR MEASLES-VIRUS ANTIBODY DETECTION

被引：10

作者：

NATES, SV ^{[1
]}

REY, GY ^{[1
]}

GIORDANO, MO ^{[1
]}

ZAPATA, MT ^{[1
]}

DEPETRIS, A ^{[1
]}

BOSHELL, J ^{[1
]}

机构：

[1] MINIST SALUD,INST NACL SALUD,SANTAFE BOTOTA,COLOMBIA

来源：

RESEARCH IN VIROLOGY | 1994年 / 145卷 / 01期

关键词：

MEASLES; SERONEUTRALIZATION; DETECTION; MODIFIED ASSAY; SPECIFICITY; SENSITIVITY; PROTECTIVE ANTIBODIES;

D O I：

10.1016/S0923-2516(07)80006-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a simple, inexpensive, sensitive and specific modified seroneutralization assay for use on a wide scale to screen for the presence of measles antibodies. After only one reaction (1 h) and a 3-day incubation period at 37 degrees C, the test can be easily read using a rapid, inexpensive methylene blue/phenol staining procedure. The determination of protective antibody titres by modified and standard seroneutralization methods was strongly correlated (corr. coff. = 0.98).

引用

页码：45 / 49

页数：5

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