PLEIOTROPIC EFFECTS OF POINT MUTATIONS IN YEAST TRANSFER RNAASP ON THE BASE MODIFICATION PATTERN

被引：15

作者：

EDQVIST, J ^{[1
]}

STRABY, KB ^{[1
]}

GROSJEAN, H ^{[1
]}

机构：

[1] LAB ENZYMOL GIF SUR YVETTE, CNRS, F-91198 GIF SUR YVETTE, FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 03期

关键词：

D O I：

10.1093/nar/21.3.413

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The base-modification pattern has been studied in several synthetic variants of yeast tRNA(Asp) injected into Xenopus laevis oocytes. Certain point mutations in the D-stem and the variable loop of the tRNA led to considerably decreased levels of m1G37, psi40 and Q34/manQ34 in the anticodon stem or loop and an increased rate of synthesis for m5C49 in the T-stem. The formation of M2G6 in the aminoacyl-stem was not affected in any of the tRNA-variants. Thus, mutations in one part of the tRNA-molecule can have long-range effects on the interactions between another part of the tRNA and the tRNA modifying enzymes.

引用

页码：413 / 417

页数：5

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