CHARACTERIZATION OF NUCLEAR MATRIX PROTEINS OF PHYSARUM-POLYCEPHALUM AND MAMMALIAN-CELLS

Analysis of the nuclear matrix of Physarum polycephalum and renal epithelial cells in culture (LLC-PK1) reveals a complex protein pattern. Applying various experimental protocols we observe only negligible differences in the final nuclear matrix protein pattern, in Physarum as well as in LLC-PK1 cells. Immunoblotting with a variety of antibodies against intermediate filament proteins and with antinuclear autoantibodies demonstrates the presence of intermediate filament proteins as components of the nuclear matrix. Preparation of type I and type II matrix structures does not yield different protein compositions, neither in Physarum nor in differentiated LLC-PK1 cells; therefore in both systems a distinction between these two types of matrices is questionable. © 1992 Academic Press Ltd.