DODECA SATELLITE - A CONSERVED G+C-RICH SATELLITE FROM THE CENTROMERIC HETEROCHROMATIN OF DROSOPHILA-MELANOGASTER

被引：107
作者：
ABAD, JP
CARMENA, M
BAARS, S
SAUNDERS, RDC
GLOVER, DM
LUDENA, P
SENTIS, C
TYLERSMITH, C
VILLASANTE, A
机构：
[1] UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOLEC,CANTOBLANCO,E-28049 MADRID,SPAIN
[2] UNIV AUTONOMA MADRID,CSIC,CTR NACL BIOTECNOL,E-28049 MADRID,SPAIN
[3] UNIV AUTONOMA MADRID,FAC CIENCIAS,DEPT BIOL,E-28049 MADRID,SPAIN
[4] UNIV DUNDEE,DEPT BIOCHEM,CANC RES CAMPAIGN,CELL CYCLE GENET GRP,DUNDEE DD1 4HN,SCOTLAND
[5] UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 10期
关键词：
CENTROMERIC SATELLITE; DROSOPHILA YEAST ARTIFICIAL CHROMOSOME LIBRARY; INSITU HYBRIDIZATION;
D O I：
10.1073/pnas.89.10.4663
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G + C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.
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页码：4663 / 4667
页数：5
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