A HUMAN SYNAPTIC VESICLE MONOAMINE TRANSPORTER CDNA PREDICTS POSTTRANSLATIONAL MODIFICATIONS, REVEALS CHROMOSOME-10 GENE LOCALIZATION AND IDENTIFIES TAQI RFLPS

A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-55 1; (1992) Proc. Natl. Acad. Sci. USA 89,10993-109971. The hSVMT shares 92% amino acid identity with the rat sequence, but displays one less consensus site for asparagine N-linked glycosylation and one more consensus site for phosphorylation by protein kinase C. The human SVMT gene maps to chromosome 10q25 using Southern blotting analysis of human/rodent hybrid cell lines and fluorescent in situ hybridization approaches. The cDNA, and a subclone, recognize TaqI polymorphisms that may prove useful to assess this gene's involvement in neuropsychiatric disorders involving monoaminergic brain systems.