TRANSLATION INITIATION REQUIRES THE PAB-DEPENDENT POLY(A) RIBONUCLEASE IN YEAST

被引：183

作者：

SACHS, AB ^{[1
]}

DEARDORFF, JA ^{[1
]}

机构：

[1] WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA 02142

来源：

CELL | 1992年 / 70卷 / 06期

关键词：

D O I：

10.1016/0092-8674(92)90246-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Messenger RNA translation initiation and cytoplasmic poly(A) tail shortening require the poly(A)-binding protein (PAB) in yeast. The PAB-dependent poly(A) ribonuclease (PAN) has been purified to near homogeneity from S. cerevisiae based upon its PAB requirement, and its gene has been cloned. The essential PAN1 gene encodes a 161 kd protein organized into distinct domains containing repeated sequence elements. Deletion analysis of the gene revealed that only one-third of the protein is needed to maintain cell viability. Conditional mutations in PAN1 lead to an arrest of translation initiation and alterations in mRNA poly(A) tail lengths. These data suggest that PAN could mediate each of the PAB-dependent reactions within the cell, and they provide evidence for a direct relationship between translation initiation and mRNA metabolism.

引用

页码：961 / 973

页数：13

共 32 条

[1] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].