SUPEROXIDE RADICAL AS ELECTRON-DONOR FOR OXIDATIVE-PHOSPHORYLATION OF ADP

被引：44
作者：
MAILER, K
机构：
[1] Saint Mary's University, Halifax
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 01期
基金：
加拿大自然科学与工程研究理事会;
关键词：
D O I：
10.1016/0006-291X(90)91240-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
When isolated rat heart mitochondria are subject to xanthine/xanthine oxidase generated free redicals, nmol quantities of ADP are phosphorylated to ATP. This effect is proportional to xanthine oxidase concentration, and is relatively independent of ADP concentration. Exogenous superoxide dismutase partially suppresses the phosphorylation. Micromolar concentrations of iron salts completely eliminate the phosphorylation. Catalase has no effect. The likely electron source, then, is superoxide radicals. The reduced minus oxidised spectra of superoxide-bombarded mitochondria show that superoxide enters the electron transport chain by reducing cytochrome c and complex IV. Mitochondria retain their ability to phosphorylate ADP in more traditional ways under the experimental conditions described. Superoxide under physiological conditions in vivomay be a source of electrons for the oxidative phosphorylation of ADP. © 1990.
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页码：59 / 64
页数：6
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