A NOVEL POLYCLONAL ANTIBODY (CL-B1/29) FOR IMMUNOLOCALIZATION OF TRANSFORMING GROWTH-FACTOR-BETA-2 (TGF-BETA-2) IN ADULT-MOUSE

被引：22
作者：
KSANDER, GA [1 ]
GERHARDT, CO [1 ]
DASCH, JR [1 ]
ELLINGSWORTH, LR [1 ]
机构：
[1] CELTRIX LABS, DEPT IMMUNOL, PALO ALTO, CA 94303 USA
来源：
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1990年 / 38卷 / 12期
关键词：
Adult mouse; Cytokine; Growth factor; Immunolocalization; Polyclonal antiserum; Transforming growth factor-β2;
D O I：
10.1177/38.12.2254647
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A polyclonal antibody (CL-B1/29) raised against a synthetic peptide with an amino acid sequence identical to the first 29 N-terminal residues of bovine bone-derived transforming growth factor-β2 (TGF-β2) was characterized and used for immunolocalization of TGF-β2 in adult mice. Reduced staining of immunoblots and tissue after absorption of the antiserum with the immunizing peptide or with TGF-β2 but not with purified TGF-β1 demonstrated that the reagent is specific for TGF-β2, with little or no crossreactivity with TGF-β1. The immunolocalization of TGF-β2 was investigated in formalin-fixed, paraffin-embedded cultured cells and murine tissue. Specimens pre-digested with testicular hyaluronidase demonstrated immunostaining predominantly of extracellular connective tissue matrix, whereas specimens predigested with pronase E demonstrated primarily cytoplasmic staining. Immunoreactivity was widely distributed in connective tissue, muscle, adsorptive and secretory epithelia, especially of endocrine tissue, and neural tissue of adult mice.
引用
收藏
页码：1831 / 1840
页数：10
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