PRIMARY RAT HEPATOCYTES EXPRESS CYCLIN D1 MESSENGER-RNA DURING THEIR GROWTH-CYCLE AND DURING MITOGENIC TRANSITIONS INDUCED BY TRANSFORMING GROWTH-FACTOR-ALPHA

被引：8

作者：

KOCH, KS ^{[1
]}

LU, XP ^{[1
]}

LEFFERT, HL ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,CTR MOLEC GENET,LA JOLLA,CA 92093

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 204卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2430

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclin D1 messenger RNA expression was investigated in differentiated proliferation-competent primary cultures of adult rat hepatocytes. Periodic expression was observed during the 12-day growth cycle (approximate to two population doublings). Upon reaching a quiescent G(0)-state, 4-kb cyclin D1 mRNA levels were undetectable. When G(0) cultures were shifted into defined media without or with 0.2-0.8 nM TGF-alpha, conditions that reinitiate full proliferative transitions synchronously, cyclin D1 mRNA levels were elevated 1.2-4.6-fold, respectively. These findings support the hypothesis that hepatic mitogens stimulate periodic cyclin mRNA expression directly. (C) 1994 Academic Press, Inc.

引用

页码：91 / 97

页数：7

共 44 条

[1] CYCLIN AND CYCLIN-DEPENDENT KINASE-1 MESSENGER-RNA EXPRESSION IN MODELS OF REGENERATING LIVER AND HUMAN LIVER-DISEASES [J].