VOLUME-SPECIFIC AMINO-ACID-ANALYSIS - A METHOD FOR Z(A) DETERMINATION

被引：5

作者：

KWONG, PD ^{[1
]}

POUND, A ^{[1
]}

HENDRICKSON, WA ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG,HOWARD HUGHES MED INST,NEW YORK,NY 10032

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 1994年 / 27卷

关键词：

D O I：

10.1107/S0021889893012592

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A general method has been developed for determining the number of molecules per asymmetric unit, Z(a), of a protein crystal. The method, volume-specific amino acid analysis, relies on the direct measurement of the number of protein molecules in a crystal as normalized by the crystal volume. The method is accurate, straightforward to implement and sensitive enough for application to small (0.002 m3) crystals. Although the procedures described here are specific to proteins, extension to nucleic acid crystals should be possible.

引用

页码：504 / 509

页数：6

共 16 条

[1] RAPID ANALYSIS OF AMINO-ACIDS USING PRE-COLUMN DERIVATIZATION [J].

BIDLINGMEYER, BA ;

COHEN, SA ;

TARVIN, TL .

JOURNAL OF CHROMATOGRAPHY, 1984, 336 (01) :93-104

[2] AMINO-ACID ANALYSIS UTILIZING PHENYLISOTHIOCYANATE DERIVATIVES [J].

COHEN, SA ;

STRYDOM, DJ .

ANALYTICAL BIOCHEMISTRY, 1988, 174 (01) :1-16

[3] METHOD FOR DETERMINATION OF THE AMINO ACID SEQUENCE IN PEPTIDES [J].

EDMAN, P .

ACTA CHEMICA SCANDINAVICA, 1950, 4 (02) :283-293

[4] YEAST TRANSFER-RNA ASP - NEW HIGH-RESOLUTION X-RAY DIFFRACTING CRYSTAL FORM OF A TRANSFER-RNA [J].

GIEGE, R ;

MORAS, D ;

THIERRY, JC .

JOURNAL OF MOLECULAR BIOLOGY, 1977, 115 (01) :91-96

[5]

HEINRIKSON RL, 1984, ANAL BIOCHEM, V136, P65, DOI 10.1016/0003-2697(84)90307-5

[6] CRYSTAL FORMS OF LAMPREY HEMOGLOBIN AND CRYSTALLINE TRANSITIONS BETWEEN LIGAND STATES [J].

HENDRICK.WA ;

LOVE, WE ;

MURRAY, GC .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (03) :829-&

[7]

KRETSINGER RH, 1973, J BIOL CHEM, V248, P3313

[8] MOLECULAR CHARACTERISTICS OF RECOMBINANT HUMAN CD4 AS DEDUCED FROM POLYMORPHIC CRYSTALS [J].

KWONG, PD ;

RYU, SE ;

HENDRICKSON, WA ;

AXEL, R ;

SWEET, RM ;

FOLENAWASSERMAN, G ;

HENSLEY, P ;

SWEET, RW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6423-6427

[9]

KWONG PD, 1989, J BIOL CHEM, V264, P19349

[10] THE X-RAY MOLECULAR WEIGHT OF HEMERYTHRIN FROM PHASCOLOSOMA-GOULDI AND SOME NOTES ON THE OXYGENATION REACTION [J].

LOVE, WE .

BIOCHIMICA ET BIOPHYSICA ACTA, 1957, 23 (03) :465-471

← 1 2 →